The Department of the Navy is procuring sequencing reagents and library preparation kits for the U.S. Naval Medical Research Unit SOUTH. The requirements include specific Illumina MiSeq and MiniSeq platform kits capable of high-quality paired-end sequencing and low DNA input library construction. All materials must be shipped via cold chain to Callao, Peru.
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Supply and delivery of Illumina sequencing and library preparation kits, including PhiX controls and Nextera XT kits, to support next-generation sequencing projects.
This listing does not include downloadable attachments. The solicitation details may be in the description below.
Verify on SAM.govThe laboratory of the Virology Department of the U.S. Naval Medical Research Unit SOUTH (NAMRU SOUTH) is looking to purchase reagents to prepare libraries using human samples that later will be sequenced in the MiSeq and MiniSeq platforms developed by Illumina, as part of the projects of next-generation sequencing (NGS) that are being conducted at this command.
BACKGROUND The kits must be designed for library preparation and sequencing. The library preparation kit should include a variety of steps that include enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime ends for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high-quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.
REQUIREMENTS Location of work / Place of delivery Shipment to NAMRU SOUTH – Callao, Peru
deliverables schedule.
Deliverables schedule (if applicable) Materials should be shipped upon award signature. Hazardous material category. None of the items are hazardous materials. List of products FC-110-3001 PhiX control v3 kit MS-102-3003 MiSeq Reagent Kit v3 (600-cycle) FC-131-1096 Nextera XT DNA Sample Preparation Kit FC-131-2001 Nextera XT Index Kit v2 Set A (96 indexes, 384 samples) FC-420-1003 MiniSeq High Output Reagent Kit (300-cycles) FC-131-2002 Nextera XT Index Kit v2 Set B (96 indexes, 384 samples) Applicable Standards These reagents are very time-sensitive and have limited expiration dates, reason for which, vendor should inform final users of expiration dates as soon as they become available and request their approval prior to shipment. Special
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