Stellaris Microscope System With Confocal 3D-STED

(RFQ) / COMBINED SYNOPSIS-SOLICITATION Solicitation Number: 75N98026Q00857 Notice Type: Combined Synopsis Title: Stellaris Microscope System with Confocal 3D-STED, Film and Spectral Multiplexing Imaging Capabilities - Brand name or Equal Posted Date: July 22, 2026 Daylight Time Set-Aside: None NAICS Code: 334516 – Analytical Laboratory Instrument Manufacturing PSC Code: 6640 – Laboratory Equipment and Supplies 1. GENERAL INFORMATION This is a combined for commercial products prepared in accordance with the FAR Subpart 12.6, as supplemented with additional information included in this notice, and in conjunction with FAR Part 12. This announcement constitutes the only solicitation; quotations are being requested, and a separate written solicitation will not be issued. The National Institutes of Health (NIH), National Heart, Lung and Blood Institute (NHLBI), Intends to acquire one (1) microscope system, including automation, software, installation, startup, training, warranty, and manufacturer-supported service, in accordance with the

requirements set forth herein. This acquisition is being conducted as an open market purchase. The Government intends to award a Firm-Fixed-Price Purchase Order resulting from this solicitation. 2. DESCRIPTION OF REQUIREMENT 2.1

Background The NHLBI Light Microscopy Core requires acquisition of a state-of-the-art laser-scanning microscope to support advanced cellular and subcellular imaging for investigators within the NHLBI Division of Intramural Research (DIR). The proposed system will replace a 12-year-old Leica SP8 microscope, which will be traded in to offset the cost of the new instrument and ensure continuity of ongoing research projects. The new platform will provide substantial improvements in sensitivity, spatial resolution, and overall imaging performance, while expanding the Core’s ability to perform super-resolution imaging, fluorescence lifetime measurements, and highly multiplexed fluorescence imaging. This instrument will serve as a shared resource used by many laboratories within the NHLBI-DIR and, to a lesser extent, investigators across the NIH. The Leica Microsystems can provide all of the required capabilities within a single integrated system, ensuring compatibility with existing workflows, user training, and experimental protocols. The enhanced capabilities are essential to meet growing demand and to support complex, high-impact research projects requiring advanced, multi-parameter and spatially resolved imaging technologies. 2.2

Objective The

objective of this acquisition is to obtain a Microscope System with Confocal, 3D-STED, FLIM, and Spectral Multiplexing imaging capabilities that integrate all of the required imaging modalities within one unified and fully supported instrument configuration. 3. Product Specifications/Features/Salient Characteristics Required Minimum Specifications: Confocal excitation: white light laser with tunable excitation range 440–790 nm with Acousto optical beam splitter system. Prism-based fluorescence detection: prism-based spectral detection with variable emission windows to 850 nm. Detectors: five (5) Hybrid (HyD) detectors optimized for spectral detection across approximately 400–850 nm. The proposed system must provide advanced confocal imaging capability and shall include a white light laser (WLL) with continuously tunable excitation from 440–790 nm integrated with acousto-optical beam splitter (AOBS) technology. The system must incorporate prism-based spectral emission detection with fully adjustable emission bandwidth selection and detection spanning 400–850 nm, along with a minimum of five (5) Hybrid (HyD) detectors appropriately matched and optimized for the emission bandwidth being measured across the full spectral detection range to ensure maximal sensitivity and signal fidelity. The overall configuration shall maximize excitation flexibility, spectral separation capability, and detection sensitivity across diverse fluorophores. 3D STED super-resolution: pulsed depletion at 775 nm and 589 nm, with three-dimensional STED capability. The proposed system must provide fully integrated three-dimensional STED super-resolution microscopy. The system shall include pulsed depletion lasers at 589 nm and 775 nm and support depletion in both the lateral (XY) and axial (Z) dimensions. The STED implementation must include the capability for time-gated fluorescence emission detection in the nanosecond time domain to enhance resolution and suppress

background fluorescence. The STED modality must be fully integrated with confocal and FLIM imaging within a unified platform configuration. The system must support fully spectrally corrected STED