Notice of Intent to Sole Source Single-Cell RNA Sequencing Reagents

Notice of Intent to Sole Source SOLICITATION NUMBER: PCA-NIA-02902 TITLE: Single-cell RNA sequencing reagents . PRIMARY

POINT OF CONTACT: Jaddua Johnston [email protected] Phone: 240-569-0006 INTRODUCTION: THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT WITHOUT PROVIDING FOR FULL AND OPEN COMPETITION (INCLUDING BRAND-NAME). The Antibody Diversity Section at the National Institute on Aging (NIA) is investigating the hypothesis that patients with Alzheimer’s disease exhibit increased immune cell infiltration into the cerebrospinal fluid (CSF). To support this research, the laboratory will perform single-cell RNA sequencing (scRNA-seq) on cellular pellets from approximately 60 human CSF samples. This requirement is for reagents necessary to partition cells into single-cell Gel Beads-in-Emulsion (GEMs) and generate barcoded cDNA libraries for downstream RNA sequencing analysis. These reagents will enable identification of immune cell populations and characterization of transcriptional differences between healthy and disease states. NORTH AMERICAN INDUSTRY SYSTEM (NAICS) CODE and SET ASIDE STATUS The North American Industry System (NAICS) code for this requirement is 334516 – Analytical Laboratory Instrument Manufacturing, with a size standard of 1,000 employees. This acquisition is not set aside for small business. REGULATORY AUTHORITY The resultant contract will include all applicable provisions and clauses in effect through Federal Acquisition Circular (FAC) 2024-07, dated August 29, 2024. This acquisition is conducted under the procedures prescribed in FAR Part 12 – Acquisition of Commercial Products and Commercial Services and FAR Subpart 13.5 – Simplified Procedures for Certain Commercial Products and Commercial Services. STATUTORY AUTHORITY This acquisition is conducted under the authority of FAR 6.302-1 – Only One Responsible Source and No Other Supplies or Services Will Satisfy Agency

Requirements (41 U.S.C. 3304(a)(1)). DESCRIPTION OF REQUIREMENT: Purchase Single-cell RNA sequencing reagents compatible with the 10x Genomics Chromium platform, including: Library Construction Kit, 16 reactions (Part No.

1000190) GEM-X Universal 5' Gene Expression v3, 16 samples Associated shipping and handling Salient characteristics: The required reagents must meet the following minimum technical characteristics: Compatibility with the 10x Genomics Chromium Controller system currently in use at NIA Capability to partition individual cells into Gel Beads-in-Emulsion (GEMs) Support for 5’ gene expression profiling workflows Integrated single-cell barcoding for downstream RNA sequencing Validated performance for human-derived biological samples, including CSF Ability to generate high-quality cDNA libraries suitable for next-generation sequencing Quantity: Quantity 2: Library Construction Kit, 16 reactions (Part No.

1000190) Quantity 1: GEM-X Universal 5' Gene Expression v3, 16 samples Delivery Date: Delivery is required within 30 days after contract award. Delivery Location: Robert W. Maul, Ph.D. Biomedical Research Center, Room 06C009 251 Bayview Blvd. Baltimore, MD 21224

PERIOD OF PERFORMANCE: Not applicable. This is a supply purchase. Standard manufacturer warranty provisions shall apply. CONTRACTING WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND-NAME) DETERMINATION The determination by the Government to award a contract without providing for full and open competition is based upon the market research conducted. Specifically, 10X Genomics. is the only vendor in the marketplace that can provide the services required by NIA. The equipment is proprietary to its manufacturer, Interaxon Inc, and this company is the sole provider of maintenance for this equipment. In accordance with FAR part 10, extensive market research was conducted to reach this determination. Additionally, contacts with knowledgeable individuals both in industry and Government, as well as reviews of available product literature, revealed no other sources. Finally, a review of the GSA Advantage, Dynamic Small Business Search, and previous government acquisitions returned no results that meet all of the